Titre : | N-cadherin activation substitutes for cell-cell contact control of myogenic differentiation (abstract : congrès international de Myologie, 2005) |
contenu dans : | |
Auteurs : | Congrès international de myologie 2005 (International Congress of Myology 2005; 9-13 mai 2005; Nantes, France) ; Mège RM ; Gavard J ; Marthiens V ; Lambert M |
Type de document : | Article |
Année de publication : | 2005 |
Pages : | p. 74 |
Langues: | Anglais |
Mots-clés : | colloque ; culture cellulaire ; différenciation musculaire ; facteur Myf-4 ; muscle squelettique ; myoblaste ; myogenèse ; N-cadhérine ; souris ; troponine T |
Résumé : |
Communication n° 241. N-cadherin is expressed throughout skeletal myogenesis, and has been proposed to be involved in the differentiation program of myogenic precursors. We further characterized the N-cadherin involvement and its mechanism of action at the onset of differentiation, through controlled N-cadherin activation, by plating isolated C2 myoblasts on surfaces coated with a chimeric Ncad-Fc homophilic ligand (N-cadherin ectodomain fused to immunoglobulin G Fc fragment). We show that N-cadherin activation may account for the cell density effect in myogenic differentiation by promoting myogenin and troponin T expression. In addition, N-cadherin adhesion participates to the associated cell-cycle arrest through the nuclear accumulation of the cyclin-dependent kinase inhibitors, p21 and p27. Mouse primary myoblast cultures exhibited similar responses to N-cadherin as C2 cells. RNA interference knockdowns of the N-cadherin-associated cytoplasmic proteins p120 and beta-catenin produced opposite effects on the differentiation pathway. p120 silencing resulted in a decreased myogenic differentiation, associated with a reduction in cadherin-catenin content, which may explain its action on myogenic differentiation. Beta-catenin silencing led to a stimulatory effect on myogenin expression, without any effect on cell-cycle. Our results demonstrate that N-cadherin adhesion substitutes for cell-cell contact-dependent cell-cycle arrest and differentiation of myogenic cells, involving regulation through p120 and beta-catenins. - Lambert M.; Choquet D. and Mège R.M., J. Cell. Biol. 2002, 157: 469-479. - Gavard J.; Lambert M.; Grosheva I.; Marthiens V.; Irinopoulou T.; Riou J-F.; Bershadsky A. and Mège R.M., J.Cell Sci. 2004, 117: 257-270. - Gavard J.; Marthiens V.; Monnet C.; Lambert M. and Mège R.M., J.Biol.Chem. 2004, 279: 36795-36802. |