Titre : | Metabolic and contractile plasticity of striated muscle cells in vitro (abstract : congrès international de Myologie, 2005) |
contenu dans : | |
Auteurs : | Congrès international de myologie 2005 (International Congress of Myology 2005; 9-13 mai 2005; Nantes, France) ; Peltzer J ; Musa H ; Peckham M ; Keller A |
Type de document : | Article |
Année de publication : | 2005 |
Pages : | p. 75 |
Langues: | Anglais |
Mots-clés : | bêta-énolase ; bêta-oxydation des acides gras ; biologie moléculaire ; chaîne lourde de la myosine ; colloque ; contractilité ; culture cellulaire ; fibre musculaire striée ; in vitro ; métabolisme énergétique ; muscle squelettique ; myogenèse ; souris |
Résumé : |
Communication n° 273. Introduction : The regulation of striated muscle plasticity and its involvement in coordination of energy metabolism and contractile phenotype remain obscure. Using an animal model for reduced contractile activity, we previously described the metabolic changes that occur in rat hindlimb muscles by analysis of twelve markers at the protein and transcript levels. These changes were adaptated to contractile modifications (1) Objectives : To further relate changes in energy metabolism to changes in contractile function, we are now studying modifications in expression of numerous selected markers, as a function of differentiation stage using an in vitro model of striated muscle plasticity. Methods : Muscle cell lines derived from the transgenic mice H2kb-tsA58 develop a sarcomeric apparatus expressing fast and/or slow adult myosin isoforms in culture (2,3), contrary to commonly used cell lines. We characterized differentiation using the following markers: myosin heavy chain (MHC) isoforms, the glycolytic enzyme beta enolase, and oxidative enzymes (beta oxidation of lipids: hydroxylCoA dehydrogenase (HAD), citrate synthase (CS)). We also studied the expression of two transcription factors, HIF-1alpha and PGC-1alpha, that play a major role in metabolic plasticity. Results : Western Blot analyses revealed a clone expressing the slow MHCI and not the fast MHC2b isoform. Changes in the phenotype of this clone occurring in modified differentiation medium (serum content, matrigel) are being characterized (Western Blot, immunocytochemistry). Transcript analyses by real time RT-PCR are on the way, using primers corresponding to 15 markers. Conclusion : By this approach, we expect to characterize new factors controlling metabolic and contractile plasticity of striated muscle cells, leading to applications in the field of muscle repair and myopathies. 1.Peltzer J (2003) DEA, Université Paris V. 2. Morgan et al(1994) Dev Biol 162, 486. 3. Miller et al (2003).J Physiol 548, 167. |