Titre : | Real time imaging of myoblast transplantation using the human sodium iodide symporter (hNIS) (abstract : congrès international de Myologie, 2005) |
contenu dans : | |
Auteurs : | Congrès international de myologie 2005 (International Congress of Myology 2005; 9-13 mai 2005; Nantes, France) ; Bouchentouf M ; Benabdallah BF ; Dumont M ; Tremblay J |
Type de document : | Article |
Année de publication : | 2005 |
Pages : | p. 103 |
Langues: | Anglais |
Mots-clés : | colloque ; dystrophie musculaire de Duchenne ; étude quantitative ; fibre musculaire striée ; humain ; in vitro ; myoblaste ; myotube ; plasmide ; souris mdx ; thérapie cellulaire |
Résumé : |
Communication n° 39. BACKGROUND : The quantification of the graft success is a key element to evaluate the efficiency of cellular therapies for several pathologies such as Duchenne muscular dystrophy. This study describes a novel approach to evaluate the success of myoblast transplantation (i.e., the survival or the transplanted cells and of the muscle fibers that they have formed) by real-time imaging. METHODS : C2C12 myoblasts were first transfected with a plasmide containing the human sodium iodide symporter (hNIS) gene. The specific uptake of 99mTCO4 by the hNIS positive myoblasts was demonstrated in vitro, no accumulation of 99mTCO4 was observed within the control cells. The cells were then transplanted into the Tibialis anterior (TA) muscle of mdx mice. Following intra-peritoneal administration of Na99mTCO4, scintigraphies (g-radiographs) were performed depicting hNIS-dependent 99mTCO4 uptake within the TA. RESULTS : Preliminary experiments have demonstrated that the resolution of the camera used is 2 mm. In vitro the cells expressing the hNIS were able to incorporate the Na99mTCO4 and were also able to form myotubes. In vivo image acquisition of transplanted mice T.A revealed that hNIS positive cells were able to incorporate the Na99mTCO4. CONCLUSIONS : This approach permitted to evaluate the progression of the transplantation and the graft success without having to biopsy the animals during the follow-up period. |