Résumé :
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Communication n° 540. INTRODUCTION : Stable gastric pentadecapeptide BPC 157 (GEPPPGKPADDAGLV, M.W. 1419 (PL-10, PLD-116, PL 14736 Pliva, Croatia)), accelerates healing of wounds, burns as well as transected Achilles tendon and shows strong anti-inflammatory effects. BPC is effective alone, without carrier, in trials for inflammatory bowel disease, wound treatment, locally or systemically, with no toxicity reported. OBJECTIVES : We hypothesize that BPC 157 may have clinical relevance as both systemic and local peptide treatment of major muscle crush injury, such as gastrocnemius muscle complex (GCC). METHODS : In crushed rats (GCC, impulse force 0.4653 Ns, kinetic energy 0.7217 J, force delivered 0.727 Ns/cm2) BPC 157 (without carrier) was applied intraperitoneally (10 µg, 10 ng, 10 pg/kg) or locally (1.0 or 0.01 µg (dissolved in distilled water)/g commercial neutral cream) (given only immediately after injury (sacrifice at 2h) and/or once daily, final 24 hours before sacrifice (at 4, 7, 14, 21, 28, 72 days)). Assessment was functional, macroscopical and histological. RESULTS : BPC 157 improves muscle healing: (i) functionally (walking test - sciatic functional index, and extensor postural thrust/motor function index show significant improvement), (ii) macroscopically (less hematoma and edema, smaller limb circumference), (iii) microscopically (less inflammation and fibrosis, more regeneration). CONCLUSION : Effective at any of the investigated intervals, BPC 157 induces post-injury muscle healing promptly and then maintains the healing with full function restoration.
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