Titre :
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Endothelial cells within embryonic skeletal muscles : a potential source of myogenic progenitors (abstract : congrès international de Myologie, 2005)
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contenu dans :
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Auteurs :
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Congrès international de myologie 2005 (International Congress of Myology 2005; 9-13 mai 2005; Nantes, France) ;
Auda-Boucher G ;
Rouaud T ;
Lafoux A ;
Huchet-Cadiou C ;
Fontaine-Perus J ;
Gardahaut M
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Type de document :
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Article
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Année de publication :
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2005
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Pages :
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p. 130
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Langues:
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Anglais
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Mots-clés :
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cellule endothéliale
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colloque
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culture cellulaire
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dystrophine
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embryogenèse
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immunohistochimie
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muscle squelettique
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myoblaste
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sarcolemme
;
souris mdx
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Résumé :
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Communication n° 249. We previously demonstrated that CD34+/Flk1+ vascular endothelial cells from the mouse embryonic muscles (17d.p.c.) have the ability to predominantly differentiate in vitro into skeletal muscle lineage and to restore dystrophin expression when injected into Tibialis Anterior muscle of mdx mouse. The results presented here confirm and extend the differentiation potential of the endothelial cells. When delivered directly into the Extensor Digitorum Longus (EDL) of mdx mice, the endothelial cells can contribute to vascularisation of host muscles. Donor-endothelial cells fuse with 70-80% of mature muscle fibers of the dystrophic host to produce hybrid fibers expressing dystrophin. Using immunohistochemical analyses we show that dystrophin is present throughout the sarcolemma of many hybrid fibers and at higher level at synaptic sites (identified by labelling of AChRs). In mdx mice, utrophin is found all along the sarcolemma. In contrast in treated mdx muscle, utrophin is confined to the neuromuscular junctions, as seen in healthy muscle. Moreover hybrid fibers isolated from transplanted mdx EDL produce donor-derived myogenic cells after 48 hours in culture which differentiate into myotubes within 5-6 days. These data suggest that injected endothelial cells have the potential to differentiate into muscle satellite cells and could maintain the satellite pool of treated muscle. One month posttransplant the recipient muscle is more resistant to fatigue during repeated maximal contractions than untreated mdx EDL muscle. Finally the incorporation of vascular endothelial cells into dystrophic fibers appears sufficient to improve muscle function of mdx muscles. The myogenic and angiogenic capacities of vascular endothelial cells within embryonic muscles open new opportunities for tissue repair.
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