Titre : | MicroRNA signature in cardiac committed human embryonic stem cells |
contenu dans : | |
Auteurs : | Congrès international de myologie 2008 (International Congress of Myology 2008; 26-30 mai 2008; Marseille, France) ; Nury D ; Barbry P |
Type de document : | Article |
Année de publication : | 2008 |
Pages : | p. 389 |
Langues: | Anglais |
Résumé : | Genetic cardiomyopathies are predominant among rare diseases. They often originate from mutations in early cardiac transcription factors. Human Embryonic stem (HES) cells represent a key developmental model which recapitulate early cardiogenesis. MicroRNAs are emerging as key regulators of transcriptional pathways, acting as inhibitors of translation initiation. Lineage specification of Human Embryonic stem cells is a tightly regulated process in which miRNAs are likely to play a key role. Using a Chip technology, we compared the expression profile of miRNAs in two distinct HES cell lines (HUES-1 and HUES24). HES cells were then committed toward a cardiac lineage using 10 ng/ml BMP2 for 48hrs. Comparison of HES cells and cardiac specified cells revealed a differential expression of 110 miRNAs (45 were up-regulated and 55 downregulated following BMP2 treatment). Chip data were validated by stem-loop real time PCR. The pattern of miRNA expression was then correlated with the one of mesodermal gene expression induced by the morphogen in both cell lines. Profile of miRNAs under basal or BMP2 conditions will help to comprehend early cardiogenesis under normal or pathological (i.e genetic disease) conditions. This work is supported by the French National Research Agency (grant Blanc06-28138290). |