Résumé :
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Myogenic progenitor cells are promising tools for cell therapy to treat neuromuscular disorders. But a challenging question is their amplification to a satisfying scale for clinical applications. Until now, most of myogenic progenitor cells have been cultivated on plastic surfaces as they are naturally adherent cells. The scaling up of such procedures results in the multiplication of culture surface (eg. Cell Factories), which will be rapidly limited. As myogenic progenitor cells are not able to grow in suspension, we have decided to test an alternative strategy to grow adherent cells in suspension: use of microcarriers. Human myoblasts (CHQ5B) have been chosen as a study model. Different microcarriers (Cytodex, Cultisphers, …) have been tested in order to find the surface best adapted for cell growth. The selected microcarriers (Cultisphers) are entirely made from gelatine and thus can be degraded by an enzymatic means (trypsine, collegenase…) and cells are collected by a simple centrifugation step. Preliminary results indicate that by using a 50ml spinner flask, an amplification of 1 week allows the production of 15.106 cells (starting with an inoculum of 2.5.106 cells). Further studies will address scale-up issues for getting 108 cells / spinner run. These amplified cells are characterized for their myogenic potential.
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