Titre :
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Real time fluorescence imaging of whole mouse muscle during eccentric exercise in vivo and in vitro : a new tool to study sarcolemmal disruption (poster)
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contenu dans :
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Auteurs :
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4th International Congress of Myology, 4ème colloque international de Myologie (9-13 mai 2011; Lille (France)) ;
Borel P ;
Guerchet N ;
Tanniou G ;
Bloch R ;
Roche J ;
Richard I ;
Stockholm D
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Type de document :
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Article
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Année de publication :
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2011
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Pages :
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p. 136
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Langues:
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Anglais
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Mots-clés :
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colloque
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Résumé :
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Sarcolemmal disruptions in myofibers can represent a physiologic response to a particular mechanic stress like lengthening contractions. These membrane tears result in intracellular changes that play a crucial role in physiopathology of the diseases related to membrane defects. A consequence of membrane injuries is an enhanced permeability to macromolecules that are present in the direct surroundings of the fibers. This uptake can easily be detected and quantified either by classical histological analysis or by real-time fluorescence imaging. In this work, we have built two systems that allow real-time fluorescence imaging of whole mouse muscle during eccentric exercise induction, both for in vivo or in vitro studies.Our first experimental set-up consists in combining a dynamometer generating eccentric exercise on the tibialis anterior (TA) muscle of a living mouse with a macroconfocal microscope. Fluorescent dyes are injected intraperitonealy before inducing lengthening contractions. Fluorescent dyes uptake in damaged myofibers can be followed on a large field of the TA with good spatial resolution but the bulging shape of muscle surface remains limiting as well as depth information that cannot be achieved easily.To overcome such critical point, a similar approach was finally developed in vitro for isolated extensor digitorum brevis (EDL) muscle. The device relies on a controlled stimulating unit enabling the muscle contraction while it is lengthened simultaneously. Muscle is soaked into a chamber perfused with oxygenated medium containing fluorescent dyes under the objective of the macroconfocal. In this case, muscle thickness and planar shape both promote interesting access to deep fibers.Such a development should help to give new insights on the dynamics of the mechanisms of membrane disruption process and repair in a context of sarcolemmal deficiencies.
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