Titre : | Conserved functions of Pax3/7 during evolution |
Revue : | Developmental biology, 344, 1 |
Auteurs : | Hayashi S ; Drayton B ; Aurade F ; Rocancourt D ; Buckingham M ; Relaix F |
Type de document : | Article |
Année de publication : | 2010 |
Pages : | p 528 |
Langues: | Anglais |
Mots-clés : | recherche biomédicale |
Résumé : |
Abstract 405:
Pax genes encode evolutionarily conserved transcription factors that play critical roles in development. Among these, the Pax3 and Pax7 genes arose by duplication from a unique ancestral Pax3/7 gene, and have similarities in their protein sequence and expression. Previously, we replaced Pax3 with Pax7 followed by an IRES-nlacZ reporter, using gene targeting in the mouse. Pax7 can substitute for Pax3 function in dorsal neural tube, neural crest cell, and somite development, but not in the formation of muscles involving long-range migration of muscle progenitor cells. We have now generated Pax3LampreyPax37-IRES-nlacZ/+ and Pax3AmphioxusPax37-IRES-nLacZ alleles in which mouse Pax3 was replaced by Lamprey or Amphioxus Pax37. Analysis of Pax3LampreyPax37-IRES-nlacZ/+ and Pax3AmphioxusPax37-IRES-nLacZ embryos reveals that Lamprey and Amphioxus Pax37, similar to mouse Pax7, can compensate for Pax3 deficiency in dorsal neural tube, and somite development. Surprisingly, muscle progenitor cells migrate to hind limb buds, though Lamprey and Amphioxus do not form limbs. Our results suggest that the current functions of these factors were already present in the Pax3/7 protein before gene duplication at the onset of vertebrate evolution. |
Pubmed / DOI : | DOI : 10.1016/j.ydbio.2010.05.386 |