Résumé :
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Communication n° 465 Limb Girdle Muscular Dystrophies (or LGMD2) are a group of autosomal recessive dystrophies characterised by the specific atrophy of muscles from the scapular and pelvic girdles. Mutations affecting 10 different proteins have been reported to be associated with LGMD2. An extensive knowledge of the biological roles of these proteins is necessary for the identification of new therapeutic targets. Understanding the mechanisms of protein interactions is one of the ways to address this question. In this study, the yeast double hybrid method was used to realise an interactome of 7 muscle proteins of which 4 are implicated in LGMD2. This screen was realised by a company specialising in these technologies (Hybrigenics). After cloning and expression into yeast, a mating was realised with yeast expressing a foetal and adult muscle tissue bank. The interactions are affected with a PBS score (Predicted Biological Score) representing a probability of existence of the interaction and a SID (Selected Interaction Domain) representing the prey minimal domain of interaction. For every protein tested, a minimum of 20 interactions were identified and are currently validated by immunoprecipitation or pull-down assays. Once confirmed, the interactions would pinpoint the cell events involving the proteins mutated in LGMD2 and could in fine be the starting point of new therapeutic solutions. If this method was to be used for a larger scale screen, this could benefit every field of fundamental and clinical research on the muscle tissue.
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