Résumé :
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Communication n° 423 Various mutants of the human anti-human-acetylcholine receptor (AChR) antibody IgG1-637 were produced and characterised. A monovalent version of this AChR antibody was created by deleting four amino acids in the CDR3 of one heavy chain. The monovalent antibodies were isolated using a His6 and a Flag tag linked to the different heavy chains. Moreover, an IgG4 isotype of the AChR antibody, which does not bind complement, was produced and characterised. The results indicate that the recombinant antibodies can compete with the IgG1 isotype and therefore might be useful for the development of immunotherapies for myasthenia gravis.
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