Titre :
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PAX7-expressing satellite cells are indispensable for adult skeletal muscle regeneration
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contenu dans :
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Auteurs :
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4th International Congress of Myology, 4ème colloque international de Myologie (9-13 mai 2011; Lille (France)) ;
Galy A ;
Sambasivan R ;
Yao R ;
Kissenpfennig A ;
Van Wittenberghe L ;
Paldi A ;
Gayraud-Morel B ;
Guenou H ;
Malissen B ;
Tajbakhsh S
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Type de document :
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Article
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Editeur :
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AFM-TELETHON, 2011
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Pages :
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p. 141
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Langues:
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Anglais
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Résumé :
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INTRODUCTION: Muscle-derived cells are able to differentiate towards osteogenic, chondrogenic or adipogenic lineage, in addition of their myogenic potential. This raises many biological and clinical questions. The cellular bases and the role of this multipotentiality are unclear. The impact for cell therapies is unknown especially regarding the choice of the best muscle cell sub-populations to regenerate dystrophic muscles or other damaged tissues. Considering the importance of intra-muscular adipocyte depositions encountered in several neuro-muscular disorders, we study the relations between myogenic and adipogenic lineages in human skeletal muscle. METHODS: Cells were isolated from muscle samples obtained as res nullus from surgeries or from diagnostic biopsies and sorted with immuno-magnetic beads or flux cytometry.RESULTS: Using the stem cell marker CD34, we were able to sort a myo/adipogenic CD34+ population and an only myogenic CD34- population on the basis of in vitro differentiation and in vitro engraftment into mouse regenerating muscles invaded by fat deposition. To obtain this fat deposition muscle fibers were injured by injection of a glycerol solution. We fractionated further the CD34+ population and found that it contains an adipogenic cell subset displaying a CD34+CD15+CD56- immunophenotype and a myogenic cell subset displaying a CD34+CD15-CD56+ immunophenotype. We present evidence that these two subsets derive from a common myo/adipogenic CD34+CD15+CD56+ subpopulation. The adipogenic and myogenic markers expressed by these populations as well as their anatomical localisation indicate that the CD34+ cells are different from the satellite cells which present a CD34- immunophenotype.CONCLUSION: These data show that an adipogenic lineage is resident in human skeletal muscle. It may be at the origin of adipocytes found in dystrophic muscles and its exact role remains to be elucidated. The identification of the CD34- purely myogenic population strongly establishes this population as a potential new tool for myoblast-based therapy of neuromuscular disorders to avoid any risk of differentiation in an undesirable adipogenic lineage.
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