Titre :
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Role of the MyoD/NFATc2 complex into myofiber formation and specification during mouse myogenesis
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Auteurs :
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Xèmes Journées Annuelles de la Société française de Myologie (SFM) (14-16 novembre 2012; Grenoble (France)) ;
Daou N ;
Lécolle S ;
Baghdadi M ;
Saclier M ;
della Gaspera B ;
Charbonnier F ;
Chanoine C ;
Armand AS
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Type de document :
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Article
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Editeur :
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Société Française de Myologie SFM, 2012
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Pages :
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p. 37
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Langues:
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Anglais
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Résumé :
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Calcineurin/NFAT (Nuclear Factor of Activated T-cells) signaling is involved in multiple aspects of skeletal muscle development and disease. A number of studies demonstrate that calcineurin plays a regulatory role in skeletal muscle adaptation and muscle regeneration by its ability to promote myotube differentiation. Calcineurin dephosphorylates members of NFAT transcription factors allowing NFAT translocation into the nucleus where it cooperates with other transcription factors to induce transcription of target genes. We demonstrated that calcineurin/NFATc3 signaling is required for primary myogenesis by transcriptional cooperation with the basic helix loop helix transcription factor MyoD (Armand et al., 2008) We now show that the NFATc2 isoform is also able to physically interact with MyoD. However, NFATc2 and MyoD interaction is unique, since the interacting domains are different from those involved in the NFATc3/MyoD interaction. The biological significance of such NFATc2/MyoD interaction is completely unknown and is likely crucial as myod:nfatc2 double deletion is lethal. Our in vitro and in vivo studies show that MyoD and NFATc2 cooperate to regulate muscle fiber formation and specification during secondary myogenesis: we have indeed revealed a significant muscle aplasia in the double mutant nfatc2/myod embryos and a specific fall of expression of the neonatal myosin heavy chain (MHC) in double mutants. Altogether these data show that NFATc2 and MyoD cooperate in vivo during late myogenesis. Now, using microarray strategy, we focus on the target genes regulated by the NFATc2/MyoD complex. Armand et al. 2008. J Biol Chem. 283 : 29004-10
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Voir aussi :
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